STATE RANKING OF CHEMISTRY OLYMPIAD,2012

STATE LEVEL CHEMISTRY OLYMPIAD 2012

MERIT LIST

CLASS: IX (J1)

Rank	Name	School	Marks
1	Ieline Ahmed	Little Flowers’ School, Nalbari	86
2	Kaustav Kashyap Das	Sankardev High School, Dispur	82
3	Pratyai Sarma	St. Theresa’s School, Morigaon	80
4	Gyandeep Daimari Kapil Ramchiary Chiranjeeb Haldar	Arunodoi Academy, Tangla Do Indus Academy, Jagiroad	78
5	Deep Anand Basumatary Ashish Jyoti Boruah Ashish Jyoti Boruah	Arunodoi Academy, Tangla Shankardev Vidya Niketan. Dhemaji Shankardev Vidya Niketan. Dhemaji	76
6	Abhigyan Khound JAHNABI HAZARIKA JAHNABI HAZARIKA Pranat Kashyap	Delhi Public School, Guwahati SISHU BIDYAPITH, KUWARITOL Do Maharishi Vidya Mandir-1, Guwahati	74
7	Ajan Surya Patowary Mukunda Madhav Boruah Sandeepan pal Ambarish Borah Raktim Saikia  Raktutpal Raj Sarma Upash Jyoti Bharadwaj Priyankush  Deka Debabrata Ray Mukunda Madhav Boruah	Little Flowers’ School, Nalbari Shankardev Vidya Niketan. Dhemaji DPS, Digboi Don Bosco H S School, Tezpur Do Do North East Academy, Mangaldai Sakardev Shisu Niketan, Karara Little Flower Eng. High School, Abhayapuri Shankardev Vidya Niketan. Dhemaji Do	72
8	Pratyush Pran Sarma Anurag Ray Arghyadeep Sur Tapash Kalita Sajid Ahmed Zahid	Little Flowers’ School, Nalbari Delhi Public School, Digboi Do Udayachal Adarsha Vidya Mandir, Mirza Little Flower Eng. High School, Abhayapuri	70
9	Kaustabh Saikia Chad Samiran Neog Nihar Jyoti Deka Sparsh Tiwari Chinmoy Tamuli	Jonaki Sangha Vidyalaya, Jorhat Salt Brook School, Dibrugarh Sakardev ShisuNiketan, Karara Little Flower Eng. High School, Abhayapuri Little Flowers’ School, Nalbari Do	68
10	Miss Mrinalini Karmakar SWABALIMA BHARALI BORUAH Rajdeep Sarma Gaurav Harlalka Rupjyoti Kalita	Salt Brook School, Dibrugarh SISHU BIDYAPITH, KUWARITOL Delhi Public School, Guwahati Happy Child High School, Guwahati Little Flowers’ School, Nalbari	66
11	Anubhav Paul Smitakshi Goswami Nilam  Rajbongshi Bankim Saikia Anirudh Goyal          Medinee Mohan Borah   Namrata  Dutta Tusher Gogoi Manash Pratim Sarma	Bongaigaon High School Salt Brook School, Dibrugarh Sakardev Shisu Niketan, Karara Navaroop Jatiya Vidyapith, Nowgong V.K.V, Tinsukia Golaghat Jatiya Vidyalaya  Shankardev Vidya Niketan. Dhemaji Shankardev Vidya Niketan. Dhemaji        G B N Academy, GU Campus	64

STATE LEVEL CHEMISTRY OLYMPIAD 2012

MERIT LIST

CLASS: X (J2)

Rank	Name	School / College	Marks
1	Arunabh Chaoudhury Ashmita Mazumdar Homanga Bharadwaj	Bajali H S School DPS, Duliajan DPS, Duliajan	98
2	Prangan Bhattacharyya Jishnav Rikhoy Hazarika Bishwajit Sarma Saurabh Kumar Sarma Vishal Raj Dutta	Little Flowers’ School, Nalbari Don Bosco Hr. Secondary School , Dibrugarh Shankardev Shishu Vidya Niketon, Bajali Sankardev Shisu Vidya Niketan, Bajali DPS, Duliajan	96
3	Kritartha Pratim Deka Pranjal Kishore Sarma Mausam Kalita Kaushik Choudhuri Rahul Thakuria Suman Das Hirak Jyoti Patgiri Ripunjay Das Bikash Jyoti Medhi	Little Flowers’ School, Nalbari Do Modern Eng. School, Rangia Saraswati Shiksha Niketan, Mirza Do Do  Shankardev Shishu Vidya Niketon, Bajali Do St. Theresa’s School, Morigaon	94
4	Bishal Ray Baruah Nilopam Deka Mrinmoy Deka Mustafizur Rahman Mayank Agarwal Dhirasri Kalita Abhisekh Agarwala Jyoti Raj Mahanta Hrishikesh Medhi Raktim Roychoudhury	Little Flowers’ School, Nalbari Do Do Ambikagiri Jatiya Bidyalya, Abhayapuri Happy Child High School Kanimara High School, Tihu Bajali H S School St. Theresa’s School, Morigaon DPS, Duliajan Do	92
5	Nayana Sarma Sunayan Sarma Pratisha Das Deep Jyoti Phukan Pranita Talukdar Sahruque Hussain Pincy Deka Krishna Barman Mitali Deka Subrat Kakati Alokesh Bordoloi	Little Flowers’ School, Nalbari Do Shankardev Shishu Vidya Niketon, Nagarbera Sankar Dev Bidya Niketan, Kapahera Ambikagiri Jatiya Bidyalya, Abhayapuri Do Faculty H S School, North Guwahati Talent Search Academy, Bajali Sankardev Shisu Vidya Niketan, Bajali Shankardev Shishu Vidya Niketon, Bajali St. Theresa’s School, Morigaon	90
6	Amitesh Rajkhowa Subrata Barman Kausar Hussain Parinita Gogoi Sourav Pratim Dewraja Najnin Ahmed Raina Das Sabi Biswas Pronali Das Nishant Kumar Barua Ankur Deka Pronali Das,         Arindam Deka      Debajit Kalita Raktim Prabal Das Samujwal Talukdar Sharmistha Parashar Simanta Das Trinity Koushik Maitreyee Sarma Jahnabi Borah Priyanjali Roychoudhury	St Marys N L College Little Flowers’ School, Nalbari Marian School, Barpeta Road Assam Vidyapith H.S School, Dibrugarh Sankar Dev Bidya Niketan, Kapahera Ambikagiri Jatiya Bidyalya, Abhayapuri Do Do Dandadhar Girls’ High School, Golaghat Sanskriti the Gurukul, Guwahati Faculty H S School, North Guwahati Dandadhar Girls’ High School Bajali H S School Pathshala Sikshapith Adarsha High School, Bajali Bajali H S School SSN Choukhuty, Bajali Sankardev Shisu Vidya Niketan, Bajali Bajali H S School Do St. Theresa’s School, Morigaon Sankardev Vidya Niketan, Sibsagar DPS, Duliajan	88
7	Pingal Pritam Jinmoy Jit Talukdar MANASJYOTI BORUAH Diksha Deka Hirakjit Pathak Jit Pratim  Sarma Nilotpal Bhattacharje Wasif Rahman Jyotishman Phukan Tutumani Chowdhuri Rakesh Kalita Hirak Jyoti Sarma Bhabishya Deepta Kakati Pranjal Kumar Nath	Little Flowers’ School, Nalbari Do KUWARITOL HIGH SCHOOL Modern Eng. School, Rangia Sarthebari High School Marian School, Barpeta Road Bongaigaon High School    Do Jatiya Vidyalaya, Tinsukia D.K.Bidyapeeth High School, Mirza Sankardev Shisu Niketan, Rangia Bajali Pathshala Sikshapith Adarsha High School, Bajali Sankardev Vidya Niketan, Charaibahi Do	86
8	Tinku Jyoti Talukdar Lakshya Jyoti Borah Hrishikesh Barua Prastuti Deka Dwipkowar Talukdar KaustavBharadwaj Ripan Nath Harshajyoti Pathak Chandana Patowary Sasanke Medhi Saurabh Prateem Kashyap Sarif-ul Alam Debasish Rajkonwar Ayan Jyoti Dutta Pritom Sarma Astha Borkataky	Kuwaritol High School Sankar Dev Bidya Niketan, Kapahera Do Sankardev Vidya Niketan, Barpeta Little Flowers’ School, Nalbari P.B.Kaniha Model H.S.School Sankardev Shisu Niketan, Rangia Do Sankardev Shisu Niketan, Tulsibari Talent Search Academy, Bajali Sankardev Shisu Vidya Niketan, Bajali Pathshala Sikshapith Adarsha High School, Bajali Spring Dale High School, Jorhat DPS, Duliajan Do Do	84
9	Amar Jyoti Bharali Subhra Sankar Chutia NILAKHI BORAH Ishita Saikia Padma Lochan Bora Debojit Das Jessica Bodosa Pranami kaushik,       Swarna Barhoi Niladri Suvra Chowdhury Vaishali Jain Akshay Jain         Khairul Khan Priyam Roy Bishal Raj Gayan Mrinmoy Brahma Dhitiraj Boro Brajamohan Thakuria Khushbu Nesa Loopamudra Goswami Pankaj Roy  Amlan Jyoti Borah Nilpaban Nath Yoshita Baruah	D.K. Bidyapeeth High School, Mirza B.L .B Saraswati Shishu Mandir SISHU BIDYAPITH, KUWARITOL Christ Jyoti School, Nowgong ST. Thomas English School, Nowgong Sankardev Sishu Niketon, Abhayapuri Kendriya Vidyalaya, Khanapara, Guwahati Golaghat Jatiya Vidyalaya Dandadhar Girls’ High School, Golaghat Sanskriti the Gurukul, Guwahati Happy Child High School Do Tarabari H S School, Bahari Hat, Barpeta Marian School, Barpeta Road Maharshi Vidya Mandir, Lalmati Sankardev Shisu Niketan, Rangia Do Do Do Bajali H S School Sankardev Shisu Niketan, Bajali Phaguna Rabha High School, Guwahati Spring Dale High School, Jorhat Sankardev Vidya Niketan, Charaibahi DPS, Duliajan	82
10	Solanki Chakraborty Rubina Begum Raunak Jajodia TONMOY BORTHAKUR Injimamul Islam PRANJAL  MALI Debashish Deka Aishwarjya Saikia Soumir Jyoti Duarah, Ankita Sharmah Samujjal Aich Mrigen Rabha Bithika Baishya Nilakshi Baishya Nayamina Talukdar Nitul Das Bithika Baishya Nilakshi Baishya Nayamina Talukdar Mridusmita Kalita Debasish Das Nilim Talukdar Priti Boruah Angina Saikia	Happy Child High School, Guwahati do Bongaigaon High School SISHU BIDYAPITH, KUWARITOL Kendriya Jatiya Bidyalaya KAMRUP  JATIYA  VIDYALAYA Sankar Dev Shisu Niketon Kampur H.S & M.P School Golaghat Jatiya Vidyalaya Dandadhar Girls’ High School Maharshi Vidya Mandir, Lalmati Assam Academy, Nalbari Do Do Do Holyheart School, Tamulpur Sankardev Shisu Niketan, Rangia Do Do Kanimara High School, Tihu Bajali Sankardev Shisu Vidya Niketan, Bajali Bajali H S School Cinamara Jatiya Vidyalaya Do	80

STATE LEVEL CHEMISTRY OLYMPIAD 2012

MERIT LIST

CLASS: XI (S1)

Rank	Name	School / College	Marks
1	Hashid Zaman	Don Bosco Sr. Secondary School, Guwahati	82
2	Angshuman Bhardwaj	Shrimanta Shankar Academy, Guwahati	80
3	Indrayudh Bandyopadhyay	Kendriya Vidyalaya, Khanapara, Guwahati	78
4	Raktutpal Nath Sauvik Chakraborty Bidyanka Borah	DPS, Digboi DPS, Digboi Reliance College, Golaghat	76
5	Abhilash Patowary Amlanjyoti Konwar Bitupon Bora Chandopal Saikia Deepak Kumar Prasad	DPS, Digboi D R College, Golaghat Do Do Do	74
6	Jyotishman Sarma	DPS, Dhaligaon	72
7	Abdul Hannan Binayak Deb	Darrang College, Tezpur Salt Brook Academy, Dibrugarh	70
8	Pramod Chetry Rinkle Jain	Kaliabor College	68
9	Shruti Kashyap Barasha Devi Kakuli Bepari	Salt Brook Academy, Dibrugarh Abhayeswari H.S.&.M.P School Do	66
10	Himangshu Dutta Rajarshi Boruah Sagar Bhuyan Hemanga Bhattacharyya Sukanya Ghosh Utpal Biswas Dibya Jyoti Saikia Kaushik Sarma	Pragya Academy Do Darrang College Do Salt Brook Academy, Dibrugarh Kaliabor College Do Don Bosco Sr. Secondary School, Guwahati	64

STATE LEVEL CHEMISTRY OLYMPIAD 2012

MERIT LIST

CLASS: XII (S2)

Rank			Marks
1	Sameeran Borah	Ramanujan Jr. College	88
2	Priyangshu Goswami	Don Bosco Sr. Secondary School, Guwahati	86
3	Bibhuti Handique	Cotton College, Guwahati	84
4	Baloy Jyoti talukdar Wasim Yuhana	Don Bosco Sr. Secondary School, Guwahati Cotton College, Guwahati	82
5	Piyush Agarwal Bhargav P. Bordoloi Kumar Sachin Swagat Baruah Afzal Hafiz	Ramanujan do do	80
6	Md. Arif Ahmed Arunabh Choudhury Mustakin Coudhury Samiran Gogoi Manish Das Bhargav Jyoti Thakur Aparajita Kar Shabnam Ali	Ramanujan	78
7	Rishabh Sarma Anwesha Phukan Uddipta Deuri Joydeep Medhi Deepraj Debnath Fateh Al Jawed Huda Jahnu Bordoloi Sushmita Mahanta Swagata Das Bishan Paul Kankan Jyoti Bora Parag Sankar Chaudhury Parveez Ahmed Deba Pratim Bora	Cotton College Salt Brook Academy Do Ramanujan	76
8	Pinaki Baruah Anurag Thakuria Rupam Baruah Mahesh Chaudhury Ashit Kumar Nath Manish Haloi Ranuj Kumar Mili Abhijit Bora Mayuri Rajkhowa Lakshya Jyoti Bora Julon Jyoti Hazarika Sakira Tabbasum Bora Rimli Debnath Sourav Banerjee Adree Sekhar Bora	J B College Cotton College Do Darrang College Do Salt Brook Academy Do Ramanujan Do Do Do Do Do Do Do	74
9	Manas Pratim Das Nisad Ajit Mahanta Dhanjit Kishore Nath Nirlipta Devi Partha Pratim Bora Sgirsha Jyoti Bardoloi Md Mohidul Islam Janed Essad Samridha Bhuyan Nilotpal Saikia Mrinal Das David Ray Shahrukh Zaman Siddiki Saikat Chakraborty	Don Bosco Sr. Secondary School, Guwahati Cotton College, Guwahati Do Do Ramanujan Jr College, Nawgong Do Do Do Do Do Do Do Do DPS, DuliajanS	72
10	Gourima Saikia Rahul Das Bibhakar Goswami Sudeepta Kataki Raj Kishore Hazarika Jaba Nayana Laskar Prarthana Deka Ajay Sharma Piyush chhajer Umang Agarwal Subham Todi Arnab Karmaka	J B College, Jorhat D K College, Mirza Ramanujan Jr College, Nawgong Do Do Do Do Do VKV, Tinsukia Do Do Do	70

CASEIN AND SOURING OF MILK

There are three kinds of proteins in milk: casein, lactalbumins, and lactoglobulins. All three are globular proteins, which tend to fold back on themselves into compact, nearly spheroidal units and are more easily solubilizied in water as collodial suspensions than fibrous proteins are. They are “complete proteins”, so called because they contain all the amino acids essential for building blood and tissue, and they can sustain life and provide normal growth even if they are the only proteins, but they can contain greater amounts of amino acids than the proteins in egg and meat.

Casein, the main protein in milk, is a phosphoprotein. The phosphate groups are attached to the hydroxyl groups of some of the amino acid side chains. Casein exists in milk as the calcium salt, calcium caseinate. It is actually a mixture of at least three similar proteins which differ primarily in molecular weight and the amount of phosphorus groups they contain ( α, β and κ caseins), they form a micelle, or a solublized unit. Neither the α nor the β casein is soluble in milk and neither is soluble either singly or in combination. If κ casein is added to either one, or to a combination of the two, however, the result is a casein complex that is soluble owing to the formation of the micelle.

  

Calcium caseinate has an isoelectric point of pH 4.6. Therefore, it is insoluble in solutions of pH less than 4.6. The pH of milk is about 6.6; therefore, casein has negative charge at this pH and is solubilized as a salt. If acid is added to milk, the negative charges on the outer surface of the casein micelles are neutralized (by protonation of the phosphate groups) and the neutral protein precipitates, with the calcium ions remaining in solution:

Ca-caseinate + 2H⁺  → casein + Ca²⁺

A natural example of this process occurse when milk sours. The souring of milk is an intricate process started by the action of microorganisms on the principal carbohydrate in milk, lactose. The microorganisms hydrolyse the lactose into glucose and galactose. Once galactose has been formed, lactobacilli, a strain of bacteria present in milk, convert it to the sour-tasting lactic acid. Since the production of the lactic acid also lowers the pH of the milk, the milk clots when it sours due to the precipitation of casein. The structure of lactic acid is shown below 

Many "cultured" milk products are manufactured by allowing milk to sour before it is processed. For instance, milk or cream is usually allowed to sour somewhat by lactic acid bacteria before it is churned  to make butter. The fluid left after the milk is churned is sour and is called buttermilk. Other cultured milk products include sour cream, yogurt, and certain types of cheese.

Isolation of Casein

WEIGHING OF MILK SAMPLES

ISOLATION OF CASEIN BY ADDING ACETIC ACID

SEPARATED CASEIN

BUCHNER FUNNEL

FILTRATION 

BIURET TEST

XANTHOPROTEIC TEST(AFTER ADDING NAOH)

XANTHOPROTEIC TEST

DRIED CASEIN SAMPLES 

CHEMISTRY PROJECT WORK,2012

REPORT OF A PROJECT WORK ON

ISOLATION OF CAESIN FROM DIFFERENT SAMPLES OF MILK AND ITS CHARACTERIZATION

SUBMITTED TO

The Department of Chemistry, Darrang College,

Tezpur-784001, Assam

 

For Partial Fulfillment of HS Final Year Chemistry Practical Examination 2012

SUBMITTED BY

NAME…………………………………………………………….

ROLL…………………… NO…………………………………..

                      REGISTRATION NO……………………….   OF ……………………………

                        SESSION……………………………………….

Under the Guidance of

Dr Palashmoni Saikia

Associate Professor, Department of Chemistry

Darrang College,Tezpur

Certificate

This is to certify that this dissertation titled “ISOLATION OF CASEIN FROM DIFFENET MILK SAMPLES AND ITS CHARACTERIZATION” submitted by Abagunthita Kashyap to th Department of Chemistry, Darrang College,Tezpur, was carried under my guidance and supervision during the academic year 2011-12.

Dr Palashmoni Saikia                                                                                                       Date:                                   

Associate Professor                                            

Department of Chemistry

ACKNOWLEDGEMENT

I am grateful to Almighty for giving me the inner strength and vision in my journey of life where this project work is no less a milestone.           

          First of all I thank my chemistry mentor MR. PRONIL BORA for his vital support, guidance and encouragement without which this project would not have come forth.

         A Special thanks to my teacher DR. PALASHMONI SAIKIA for his continuous support, advices and for giving useful suggestions and relevant ideas that facilitate an easy and early completion of this project

          I also express my gratitude our Lab Assistants SRI NIRANJAN DEKA, SRI PRANJAL BORAH, SRI PRATIM SAIKIA, SRI MANJUL BHUYAN AND RATUL BORAH for their willingness in providing us with necessary lab equipments and constant support without which this effort would have been worthless

          I am grateful to my parents and my brother whose blessing and wishes have gone a long way in the completion of this task

          Last but not the least I thank all my friends specially my best friend, without their prompt support my efforts would have been in vain  

                   Barasha Saharia                                                                         Dated:

 Preface

Milk is a complex biological mixture of chemicals that serves as the primary food source

for infant mammals. Milk contains most of the biological molecules necessary to sustain life including 

water, a variety of vitamins, minerals, proteins, sugars, and lipids (DNA, although necessary for life, is 

made by your body and therefore is not needed on a dietary level). Although most mammals stop 

drinking milk upon maturity, many (but not all) human cultures continue drinking milk and consuming 

milk products (for example, cheese, butter, and cream) throughout their entire life. 

It would be quite challenging to isolate every component of milk because some of the chemicals

are present in very small quantities. One can very easily, however, isolate the most abundant

components from each other. A common example of this 

is removing the fat from cow’s milk to make skim milk or 

removing the sugar from milk to make lactose‐free milk.

 The aim of this project is to isolate casein the most important protein present in milk. Through this work an attempt has been made to compare the percentage of casein present in cow,goat and buffalo milk. The isolated casein will be then characterized by biuret test and xanthoproteic test.

                                                        Content:

                                                                                                               Page No

1. Introduction

    1.1 Milk

    1.2 Composition of milk 

    1.3 Curdling of milk

    1.4 Butter and Cheese 

     1.5 Milk and health

2. Isolation of Casein

     2.1 Objective

    2.2 Methodology 

      2.3 Things required

      2.4 Procedure

      2.5 Yield

3. Characterization of casein

    3.1 Biuret test

    3.2 Xanthoproteic test

4. Conclusion

5. Reference

UGC SPONSORED NATIONAL SEMINAR ON RECENT CHALLENGES FOR CHEMICAL RESEARCH AND PRACTICES

WELCOME TO RCCRP

MESSAGE FROM THE CONVENER

National Seminar: Recent Challenges for Chemical Research and Practices:

 Moulding Chemistry towards a better tomorrow (RCCRP).

Dated: 22-10-2012

Dear Participants,

                             Thanks for your overwhelming response. We have received 73 abstracts for RCCRP. That is really encouraging for us. There will be 10 invited lectures. I welcome you all to RCCRP.

                    In view of large number of participants we are also encouraging poster presentation. Thos who are interested in POSTER presentation kindly inmate us on or before 30-10-2012. I also request the participants to design their oral presentations as short as possible.  See you all at Tezpur.

We are also going to publish the proceeding of the seminar as post publication. If you are interested you can send the full paper as soft copy.You can also submit CD of the same at the time of registration.

   Organiser                                                                                                           Yours Truly

Department of Chemistry                                                                            Dr Palashmoni Saikia Darrang College                                                                                               Convener, RCCRP

Dist-Sonitpur                                                                               E-mail:palashms@rediffmail.com

Tezpur-784001                                                                                                Mob: 9435082506

Assam, India                                    

Official blog: chemdarrang.blogspot.com